In recent years, thanks to the rapid development of the biological products industry, cell culture technology has been widely used in the fields of monoclonal antibodies, viral vaccine products and so on. Among many culture methods, cell factory culture technology is a common large-scale cell culture method with the following advantages:

With the in-depth development of life science, cell culture technology has been widely used in many fields such as biopharmaceutical, vaccine industrial production, monoclonal antibody and so on. According to the different growth modes of cells, they can be divided into adherent cells and suspended cells. Which cells are mainly suitable for the culture of cell factories?

The biopharmaceutical specialty cultivates high-tech applied talents who master the basic theories, basic skills and process engineering of modern biological science and biotechnology, have the professional quality of biopharmaceutical technology, and can be engaged in drug production, management and technology research and development. Students are required to master the basic theory, basic knowledge and basic skills in the field of biopharmaceutical; Master the process and equipment design method of drug production device; Have the preliminary ability to research, develop and design new resources, new products and new processes of drugs; Have the ability to design and implement experiments, summarize and analyze experimental results and write papers; Be familiar with the national guidelines, policies and regulations on pharmaceutical production, design, research and development, environmental protection, etc; Proficient in a foreign language, with the ability of listening, speaking, reading and writing; Master the basic methods of document retrieval and data query, and have certain scientific research and practical work ability.

Biopharmaceutical is the use of biotechnology to produce natural active substances in living organisms. Its effective ingredients are usually some bioactive proteins, DNA, viruses, cells or tissues, etc. the administration method is direct tissue injection. The preparation of drugs is very dependent on the direct culture of biological tissues / cells and usually cannot be accurately replicated.

Disposable, integrated and large-scale are the development trend of cell gene therapy production equipment and technology.

Vaccine is an automatic immune agent for the prevention of infectious diseases, which is made of pathogenic microorganisms (such as bacteria, viruses, etc.) and their metabolites through artificial virus reduction, inactivation or genetic engineering. According to the different types of infectious disease pathogens it prevents, it can be roughly divided into bacterial vaccines and viral vaccines. Many viral vaccines used in the population now need to be prepared through the process of virus culture, purification, concentration, inactivation or lysis. Because virosomes are a kind of parasitic microorganisms specialized in host cells, their reproduction needs to be carried out in their specific host cells. Therefore, the cultivation of viruses in the production of viral vaccines requires the cultivation of animal cells in which the virosomes can reproduce first.

01 introduction to biopharmaceutical Biopharmaceutical is a product for prevention, treatment and diagnosis that uses biotechnology to separate effective components from organisms, biological tissues, cells and body fluids. Biopharmaceutical is an interdisciplinary comprehensive field formed by the combination of biotechnology and the pharmaceutical industry, covering chemical pharmaceuticals, biotechnology drugs, traditional Chinese medicine, medical devices and health industries. It is one of the most active fields in the current international scientific frontier. Biopharmaceutical raw materials are mainly natural biological materials, so the safety is relatively high and the side effects are relatively small. These significant advantages make biopharmaceutical accepted by more and more people, and it is also one of the reasons for the rapid development of biopharmaceutical. At present, biopharmaceutical is the industry with the most extensive application of biotechnology, and is also considered to be one of the most promising industries in the 21st century. Biopharmaceutical products include antibodies, vaccines, blood products, cell therapy, recombinant proteins, diagnostic reagents, etc., which are mainly used for the treatment of tumors, AIDS, cardiovascular and cerebrovascular diseases, hepatitis, autoimmune diseases and metabolic related diseases.

There are many expression hosts of foreign proteins, including prokaryotic cells (E.coli), yeast cells and insect cells. However, as foreign proteins used in human drugs, mammalian cells are used as hosts for expression, which is closer to their natural conformation, such as Chinese hamster ovary cells (CHO cells). As the most widely used animal cell in bioengineering pharmacy, CHO cell has its advantages and disadvantages. At the same time, we have many points to pay attention to in the process of cell culture, which are introduced one by one.

Biological agents are not only different from general drugs in raw materials, but also in production processes. In short, it uses modern biotechnology to produce drugs with the help of some microorganisms, plants or animals. Protein or nucleic acid drugs developed by DNA recombination technology or other new biological technologies also become biological agents. Pure biological agents, that is to say, they do not contain traditional chemical drugs except excipients.

In the pharmaceutical industry, cell culture has become an important part of antibody production. Cell culture technology has been used to produce antibodies against rotavirus, poliomyelitis, smallpox, hepatitis, rubella and chickenpox. Cell based influenza antibodies have also been approved for use in the United States and many European countries. What is the prospect of cell culture?