Biopharmaceutical is the use of biotechnology to produce natural active substances in living organisms. Its effective ingredients are usually some bioactive proteins, DNA, viruses, cells or tissues, etc. the administration method is direct tissue injection. The preparation of drugs is very dependent on the direct culture of biological tissues / cells and usually cannot be accurately replicated.

Animal cell culture is to take relevant tissues from the animal body, disperse them into individual cells (using trypsin or collagenase), and then put them in a suitable culture medium to allow these cells to grow and proliferate.

When culturing a certain cell for the first time, you must first search the name of the cell to understand relevant information, including the culture medium, serum, additives, normal digestion time, passage time, etc. For specific cells (such as primary cultured cells), it is necessary to consult relevant literature to obtain more accurate culture methods.

Nutritional conditions of cell culture

Disposable, integrated and large-scale are the development trend of cell gene therapy production equipment and technology.

After the cells are inoculated or subcultured, the experimenter should make routine examination of the cells every day or at most 1 ~ 2 days. Observe the morphology and growth of cells, as well as the pH change of culture, whether there is pollution, etc. According to the dynamic changes of cells, do fluid replacement or passage treatment. If abnormal conditions are found, take measures in time.