Let's understand the specific process of cell culture medium subpackaging
In the preparation or production process of cell culture medium, the culture medium subpackaging (including manual and instrument) is to subpackage the finished culture medium after mixing all components together.
medium is an artificial nutrient for the growth and maintenance of microorganisms, plants and animal tissues. It generally contains water, nitrogen sources, inorganic salts (including trace elements), carbon sources, growth factors (vitamins, amino acids, bases, antibiotics, pigments, hormones, serum, etc.).
the temperature of agar containing medium shall be controlled at 45-48 ° C, the contact plate medium shall be sub packed for about 15 times per minute, and the sedimentation bacteria culture dish shall be sub packed for about 10-15 times per minute. Subpackaging of sterile liquid, etc. Determination of antibiotic titer, subpackaging of dishes, etc. Touch screen technology, easy to rewrite any volume, any number of copies, any time interval, any speed.
1. Pack the culture medium into triangular flasks and test tubes of different capacities as required. The amount of sub packaging should not exceed 2 / 3 of the container to avoid overflow during sterilization.
2. The sub loading capacity of agar slope is 1 / 5 of the capacity of the test tube. After sterilization, it must be placed into a slope while hot, and the slope is about 2 / 3 of the length of the test tube
3. The semi-solid sub loading capacity is about 1 / 3 of the length of the test tube. After sterilization, it is set upright for medical use.
4. The subpackaging capacity of high-level agar is about 1 / 3 of that of the test tube. After sterilization, stand upright while it is hot, and solidify when it is cold.
5. The liquid is sub packed in the test tube, which is about 1 / 3 of the length of the test tube.
6. Cool the sterilized (or heat melted) culture medium to about 50 ℃, pour it into the sterilization plate through aseptic procedures, pour about 13-15ml of culture medium into the plate with an inner diameter of 9cm, gently shake the bottom of the plate to lay the culture medium flat on the bottom of the plate, and it will be ready after solidification. When pouring the culture medium, do not open all the lid of the plate to avoid dust and bacteria in the air.
the newly made plate culture medium (hereinafter referred to as the plate) has a lot of surface moisture, which is not conducive to the separation of bacteria. Generally, the plate should be placed upside down in the 37 ℃ incubator for about 30 minutes and used after the plate is dried.
culture medium subpackaging is widely used in microbial culture and identification, tube subpackaging, EP / centrifuge tube subpackaging, various types of porous plates, Drosophila culture medium dispensers, diagnostic reagents, and the size of the workbench can be customized to meet the needs of various liquid subpackaging.