Let's learn about the specific process of cell culture medium subpackaging
During the preparation or production of cell culture medium, the sub packaging of culture medium (including manual and instrument) is to mix all components together and then sub package the finished culture medium.
medium is an artificially prepared nutrient for the growth and maintenance of microorganisms, plants and animal tissues. It generally contains water, nitrogen source, inorganic salts (including trace elements), carbon source, growth factors (vitamins, amino acids, bases, antibiotics, pigments, hormones, serum, etc.).
the temperature of agar containing medium shall be controlled at 45-48 ° C. about 15 pieces of contact dish medium shall be sub packed for 1 minute, and about 10-15 pieces of sedimentation bacteria culture dish shall be sub packed for 1 minute. Subpackage of sterile liquid, etc. Antibiotic titer determination, plate sub packaging, etc. Touch screen technology is convenient to rewrite any volume, any number of copies, any time interval and any speed.
1. Pack the culture medium into triangular flasks and test tubes with different capacities as needed. The amount of sub packaging should not exceed 2 / 3 of the container to avoid overflow during sterilization.
2. The sub loading capacity of agar inclined plane is 1 / 5 of the test tube capacity. After sterilization, it must be placed into an inclined plane while hot. The length of the inclined plane is about 2 / 3 of the length of the test tube
3. The sub packaging amount of semi-solid is about 1 / 3 of the length of the test tube. After sterilization, it is solidified vertically for medical use.
4. The sub loading capacity of high-level agar is about 1 / 3 of the test tube. After sterilization, stand upright while hot and solidify after cold.
5. The liquid is sub packed in the test tube, which is about 1 / 3 of the length of the test tube.
6. Cool the sterilized (or heated and melted) medium to about 50 ℃, pour it into the sterilized plate through aseptic procedures, pour about 13 ~ 15ml of the medium into the plate with an inner diameter of 9cm, gently shake the bottom of the plate to lay the medium on the bottom of the plate, and it will be ready after solidification. When pouring the medium, do not open all the plate covers to avoid dust and bacteria in the air.
the newly made plate culture medium (referred to as plate for short) has a lot of surface moisture, which is not conducive to the separation of bacteria. Generally, the plate should be put upside down in the 37 ℃ incubator for about 30 minutes and used after the plate surface is dried.
culture medium subpackaging is widely used in microbial culture and identification, test tube subpackaging, EP / centrifuge tube subpackaging, various types of porous plates, fruit fly culture medium distributor, diagnostic reagent, and the size of worktable can be specially customized to meet the subpackaging needs of various liquids.